谁使用过wu-blast

Bioedit提供了本地blast工具（菜单里的local blast 功能），如图一。你需要把基因组数据做成本地数据库，使用菜单里的create a local nucleotide database file功能建立本地DNA数据库，建库的原始数据需要做成fasta格式的，建成的数据库存放在\bioedit\database文件夹里。然后用另一种菌的基因序列和基因组本地数据库做blastn或者tblastx（图二），就能找到与这个基因序列相似性较高的contig了。我的bioedit版本较低，估计也能参考吧。也可以直接从NCBI下载本地blast相关程序，使用dos命令行 *** 作。

朋友给段代码给你，这个代码不要任何控件，你只需要把以下代码复制到你的窗体代码中就行了,代码很简单，相信你能看懂！仅仅用了一个结构（ShenTi）一个数组（ZhuangTai(23, 23) As Long） 一个变量（GFangXiang）

代码中自动生成了Timer1，Label1控件。以及几个函数（Randomize，Circle，Line，Erase...） 相信这些对于你来说很简单，只是没想到用这些方法来实现而已，现在你开始看看效果吧！

代码如下：

'贪吃蛇代码(无控件、全代码）

Private WithEvents Timer1 As Timer

Private WithEvents Label1 As Label

Dim GFangXiang As Boolean

Dim HWB As Single

Dim She() As ShenTi

Dim X As Long, Y As Long

Dim ZhuangTai(23, 23) As Long

Private Type ShenTi

F As Long

X As Long

Y As Long

End Type

'按键反应 ←↑↓→

Private Sub Form_KeyDown(KeyCode As Integer, Shift As Integer)

Dim C As Long

If KeyCode = 27 Then End 'ESC退出

If KeyCode = 32 Then

If Timer1.Enabled = True Then '空格暂停

Timer1.Enabled = False

Label1.Visible = True

Else '空格开始

Timer1.Enabled = True

Label1.Visible = False

End If

End If

C = UBound(She)

If GFangXiang = True Then Exit Sub

Select Case KeyCode

Case 37 '←

If She(C).F = 2 Then Exit Sub

She(C).F = 0

GFangXiang = True

Case 38 '↑

If She(C).F = 3 Then Exit Sub

She(C).F = 1

GFangXiang = True

Case 39 '↑

If She(C).F = 0 Then Exit Sub

She(C).F = 2

GFangXiang = True

Case 40 '→

If She(C).F = 1 Then Exit Sub

She(C).F = 3

GFangXiang = True

End Select

End Sub

Private Sub Form_Load()

Me.AutoRedraw = True

Me.BackColor = &HC000&

Me.FillColor = 255

Me.FillStyle = 0

Me.WindowState = 2

Set Timer1 = Controls.Add("VB.Timer", "Timer1")

Set Label1 = Controls.Add("VB.Label", "Label1")

Label1.AutoSize = True

Label1.BackStyle = 0

Label1 = "暂停"

Label1.ForeColor = RGB(255, 255, 0)

Label1.FontSize = 50

ChuShiHua '初始化

End Sub

Private Sub Form_Resize()

On Error GoTo 1:

With Me

If .WindowState <>1 Then

.Cls

.ScaleMode = 3

HWB = .ScaleHeight / .ScaleWidth

.ScaleWidth = 24

.ScaleHeight = 24

Label1.Move (Me.ScaleWidth - Label1.Width) / 2, (Me.ScaleHeight - Label1.Height) / 2

HuaTu

Me.Line (X, Y)-(X + 1, Y + 1), RGB(255, 255, 0), BF

End If

End With

1:

End Sub

Private Sub Timer1_Timer()

Dim C As Long, I As Long

On Error GoTo 2:

QingChu '清图

C = UBound(She)

Select Case She(C).F

Case 0

If ZhuangTai(She(C).X - 1, She(C).Y) = 2 Then

C = C + 1

ReDim Preserve She(C)

She(C).F = She(C - 1).F

She(C).X = She(C - 1).X - 1

She(C).Y = She(C - 1).Y

ChanShengShiWu

GoTo 1:

ElseIf ZhuangTai(She(C).X - 1, She(C).Y) = 1 Then

GoTo 2:

End If

Case 1

If ZhuangTai(She(C).X, She(C).Y - 1) = 2 Then

C = C + 1

ReDim Preserve She(C)

She(C).F = She(C - 1).F

She(C).X = She(C - 1).X

She(C).Y = She(C - 1).Y - 1

ChanShengShiWu

GoTo 1:

ElseIf ZhuangTai(She(C).X, She(C).Y - 1) = 1 Then

GoTo 2:

End If

Case 2

If ZhuangTai(She(C).X + 1, She(C).Y) = 2 Then

C = C + 1

ReDim Preserve She(C)

She(C).F = She(C - 1).F

She(C).X = She(C - 1).X + 1

She(C).Y = She(C - 1).Y

ChanShengShiWu

GoTo 1:

ElseIf ZhuangTai(She(C).X + 1, She(C).Y) = 1 Then

GoTo 2:

End If

Case 3

If ZhuangTai(She(C).X, She(C).Y + 1) = 2 Then

C = C + 1

ReDim Preserve She(C)

She(C).F = She(C - 1).F

She(C).X = She(C - 1).X

She(C).Y = She(C - 1).Y + 1

ChanShengShiWu

GoTo 1:

ElseIf ZhuangTai(She(C).X, She(C).Y + 1) = 1 Then

GoTo 2:

End If

End Select

ZhuangTai(She(0).X, She(0).Y) = 0

For I = 0 To C

Select Case She(I).F

Case 0

She(I).X = She(I).X - 1

Case 1

She(I).Y = She(I).Y - 1

Case 2

She(I).X = She(I).X + 1

Case 3

She(I).Y = She(I).Y + 1

End Select

Next

TiaoZheng

1:

GFangXiang = False

ZhuangTai(She(C).X, She(C).Y) = 1

HuaTu

Exit Sub

2: '游戏结束

If MsgBox("得分：" &UBound(She) - 2 &"分 " &vbCrLf &"游戏结束，点“是”重新开始游戏，点“否”", vbYesNo, "贪吃蛇") = vbYes Then

ChuShiHua

Else

End

End If

End Sub

'初始化

Private Sub ChuShiHua()

Me.Cls

Timer1.Enabled = True

Timer1.Interval = 50

Erase ZhuangTai

ReDim She(2)

She(0).F = 2

She(0).X = 9

She(0).Y = 11

ZhuangTai(9, 11) = 1

She(1).F = 2

She(1).X = 10

She(1).Y = 11

ZhuangTai(10, 11) = 1

She(2).F = 2

She(2).X = 11

She(2).Y = 11

ZhuangTai(11, 11) = 1

HuaTu '画图

ChanShengShiWu

End Sub

'清图

Private Sub QingChu()

Dim I As Long

For I = 0 To UBound(She)

Me.Line (She(I).X, She(I).Y)-(She(I).X + 1, She(I).Y + 1), Me.BackColor, BF

Next

End Sub

'画图 蛇

Private Sub HuaTu()

Dim I As Long

For I = 0 To UBound(She)

Me.Circle (She(I).X + 0.5, She(I).Y + 0.5), 0.49, RGB(255, 255, 0), , , HWB

Next

End Sub

Private Sub TiaoZheng()

Dim I As Long

For I = 0 To UBound(She) - 1

She(I).F = She(I + 1).F

Next

End Sub

'随机产生食物

Private Sub ChanShengShiWu()

Randomize Timer

1:

X = Int(Rnd * 24)

Y = Int(Rnd * 24)

If ZhuangTai(X, Y) >0 Then GoTo 1:

ZhuangTai(X, Y) = 2

Me.Line (X, Y)-(X + 1, Y + 1), RGB(255, 255, 0), BF

End Sub

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