用的轮子: the reel in usage 或者 the reel you are using
纺车轮:Spinning reel (Open Face Spinning reel指开放式的纺车轮)(猪嘴轮: CLOSED SPINNING REEL)
路亚轮(BAITCASTING REEL):国内习惯把 “水滴轮”和“鼓轮”都称为BAITCASTING REEL。其实“鼓轮”更为接近于海钓轮(TROLLING REEL/BIG GAME REEL)如果不好理解 rise to the bait,可以看看相似的一个习语 swallow the bait。
二者都可理解为上钩,那么 rise 的就可理解为“达到较高的某个位置”,加上 to the bait,就好理解了哈:达到成为诱饵的地步,当然就上当了哈。也可按习惯将 rise 理解为“上升、提升”,而将 the bait 用其其动词属性含义予以记忆即不断用侮辱等方式逗弄、激怒,生活中不是常常有人故意挑逗或刺激以激怒某人,使其更容易落入圈套而上当吗。但是切记这两个习语通常都不用于被动式哦。
愚人节会有什么样的英语短语我们会在那一天用到呢下面是我给大家整理的愚人节英语短语的相关知识,供大家参阅!
愚人节英语短语1例如,文章里所引用的“专家”的名字有可能就是“愚人节”这几个字的拼字游戏。
For example the name of an ‘expert’ quoted in the article might be an anagram of ‘April Fool’
愚人节是大家互相开玩笑,用假话捉弄对方节日。没人知道这个节日时怎么开始的,但是很多人认为是起源于法国。
April Fools' Day is a day to play jokes on others, No one knows how this holiday began butpeople think it first started in France
比如,一部在愚人节上映的英国短片,竟是一部非常详细的关于“种植面条的农夫”和他们如何从面条树上收割他们的面条的纪录片。
For instance, a British short film once shown on April Fool's Day was a fairly detaileddocumentary about "spaghetti farmers" and how they harvest their crop from the spaghettitrees
日前,美国“恶作剧博物馆”网站根据恶作剧的知名度、荒诞性及被愚弄的人数,评选出了媒体所制造的愚人节“十大经典恶作剧”。 其中的几个如下
Here are several of the top ten April Fool's Day pranks ever pulled off, as judged by the USwebsite of Museum of Hoaxes for their notoriety, absurdity, and number of people duped
然而,从树上长出了意大利面的电视新闻到左撇子汉堡的广告,媒体在愚人节制造的各种稀奇古怪的恶搞新闻也有一段“光辉”历史了。
However, from television revealing that spaghetti grows on trees to advertisement for left-handed burgers, the tradition of April Fool's Day stories in the media has a weird and wonderful history
日前,美国“恶作剧博物馆”网站根据恶作剧的知名度、荒诞性及被愚弄的人数,评选出了媒体所制造的愚人节“十大经典恶搞新闻”。 其中的几个如下
Here are several of the top ten April Fool's Day pranks ever pulled off, as judged by the USwebsite of Museum of Hoaxes for their notoriety, absurdity, and number of people duped
比如,在愚人节那天,曾经有一部英国短片较为详细地记录了“种意大利面的农民”,以及他们如何从意大利面树上收获意大利面。
For instance, a British short film once shown on April Fool's Day was a fairly detaileddocumentary about "spaghetti farmers" and how they harvest their crop from the spaghettitrees
伦敦城的老板们被建议取消会议而且要增加特别的安全措施在他们的办公室里以防在这个所谓的”金融愚人节”期间的抗议活动
City bosses have been advised to cancel meetings and place extra security on their offices inanticipation of trouble on what the protesters have labelled Financial Fools’ Day
和其他那些不“愚”的节日不同,愚人节(有时也叫做所有愚人的节日)的历史并没有完全被弄清楚。
Unlike most of the other nonfoolish holidays, the history of April Fool's Day, sometimes called AllFool's Day, is not totally clear
此外,可能是因为有些人的幽默感跟其他人不一样吧,因而他们把分手的日子定在了愚人节那一天。
And, possibly showing that some people's sense of humor is more twisted than others, there's a spike in breakups on April Fool's Day
你知道愚人节是怎么回事台球
A:Do you know about All Fools' Day
因此愚人节最终发展成了一个国际化的开玩笑的节日,可以说,各个国家的人都有他们专门的方式来捉弄他们的朋友和家人。
April Fool's Day thus developed into an international fun fest, so to speak, with differentnationalities specializing in their own brand of humor at the expense of their friends and families
愚人节英语短语2关于“欺骗”的单词:
1confidence,con
confidence最常用的意思是自信,也有欺骗的意思。如果你在街上有人捡了钱包要跟你分钱,这就是一个典型的confidence game,简称con game。从这个词组衍生出的con一词,可以用做动词和名词使用,也是“骗人、骗子”的意思。长线骗局叫做long con,指的是那种精心设下圈套一步一步引人上钩的。
例:He was skinned of all his money by confidence tricksters
他所有的钱都被专骗老实人的骗子骗走了。
2cheat
n 欺骗,作弊,骗子
vtvi 欺骗,逃脱,骗取
cheat是表示欺骗的最常见的词,考试作弊也用这个词。
例:He tried to tempt me to cheat in the examination
他想诱使我在考试中作弊。
3hustle
表示兜售;(靠不正当手段)赚取;骗钱等等意思。
例:He often hustles on the streets to pay for drugs
为弄到钱买毒品,他常在街上行骗。
4rip off
撕掉,扯掉;偷窃,抢走,欺骗
例:This salesman ripped us off!
这个商人欺骗了我们!
5 monkey business
我们看到有人耍小把戏,会说这个人是耍猴的,英语里面也有这种说法。monkey business有胡闹、欺骗、恶作剧等等意思。
例:After the teacher left, there was a lot of monkey business in the class 在老师离开后,班上恶作剧连连。
关于“欺骗”的句子:
1You rose to the bait
你中圈套了。
这句话rose使用的是过去式,应该是因为,你总要在别人上当了之后才对他们说这句话。
2Why should you always like to rib others
如果有人专门喜欢捉弄人,就可以对他或她说这句话,“为什么你总喜欢拿别人开心”
3Lie to me
lie to me如果作为一个短语放在句子里,通常表示某人向我撒谎、某人欺骗我的意思,但是这里作为一个整句,意思就不一样了。表达的是“放马过来!”“骗骗我试试!”如果在愚人节你对自己的防骗能力很有信心,可以自信满满地说一句“Lie to me”。
如果你非常“不幸”地在今天被“整”,那么说明你的人缘还算不错,不妨趁整你的朋友们大笑时也反整他们一次,比如装作非常生气的样子——
Eyebrows down and together + Eyes glare + Narrowing of the lips
眉头向下紧蹙 怒目瞪视 双唇紧抿
愚人节英语短语31 Today is April Fools'Day
今天是 愚人节
2 Yes, we have Thanksgiving Day, Labor Day and April Fool's Day
是的我们有感恩节 、 劳动节,以及愚人节
3 April Fool's Day is on April 1
愚人节在四月一号
4 Practical jokes are a common practice on April Fool's Day
恶作剧是愚人节的惯例
5 What ( happened to ) you on April Fool's Day
在愚人节那天你遇到了什么事
6 Haha April Fool's ! Do you forget what day it is today
哈哈今天是愚人节! 怎么都忘了
7 Today is All Fool's Day, except the fool on holiday
愚人节看起来倒像是个求爱高峰日, 有时间可以做个调查
8 At first I wondered if it was an April Fool's joke
一开始,我怀疑这是个愚人节的玩笑
9 And Americans still play tricks on each other on April Fool's Day
如今美国人仍在愚人节互相开玩笑
10 We like to make him an April fool every year
每年我们都喜欢在愚人节愚弄他
11 On April Fool's Day , be alert pranks all day long
愚人节这天一定要高度戒备起来
12 Are you fooled when it comes to another April Fool's Day
又一年的愚人节,今天你被愚乐了 吗
13 What is April Fools Day and what are its origins
什么是愚人节它的由来又是什么
14 People play practical jokes on April Fools'Day by tradition
人们习惯在 愚人节 开一些无伤大雅的玩笑
15 Do your friends play jokes on you on April Fool's Day
你的朋友愚人节跟你开玩笑 吗
You know that I want to do a thing is what I told you to fly!
I want to fly to Taiwan and little friends hug I want to fly to Grandpa Bethune's land, a look at his home I want to fly to Eagle Eagle home have a look, how much, is it right Like text introduced I want to fly to my home, ask the little swallow why will fly to the south, do not stay in my home I want to fly to the small bee family, have a look the little soldiers are in busy what I want to fly to Mars, to have a look there is no aliens, there is no life, there are no children and a happy home I want to fly to the nature, have a look the beautiful nature, to understand that all life is how you live I want to have a look can make happy people find happiness, no sadness, trouble
你知道我最想做的一件事是什么吗?我告诉你是飞翔!
我要飞到台湾和小朋友紧紧拥抱。我要飞到白求恩爷爷的国土,看一看他的老家。我要飞到老鹰的家,看看老鹰有多大,是不是像课文中介绍的那样。我要飞到小燕子的家,问一问小燕子为什么要飞到南方去,不呆在我的家。我要飞到小蜜蜂的家,看看蜜蜂小战士都在忙碌些什么。我要飞到火星上去,去看看上面有没有外星人,有没有生命,有没有小朋友和快乐的家。我要飞到大自然中去,看看美丽的大自然,去了解所有的生命都是怎么生活的。我还要看看能不能让不快乐的人寻找到快乐,没有伤心、烦恼……
How to save water
Water, is the source of human life Water conservation must starts from me, from the start, everyone is the obligation and responsibility
We should develop energetically afforestation, increase forest area, water conservation Make full use of water-saving appliances, extensive conduct propaganda water conservation benefits, make people consciously aware that water is a precious resource, abandon the "inexhaustible, be inexhaustible " stereotypes
In order to create a better environment, we should cherish every drop of water Let us start from the little things around us, start from our own, to protect the limited water resources, protect the environment on which people live I hope people can take practical action to make up for past mistakes
如何节约用水
水,是人类生命的源泉。节约用水必须由我做起,从小做起,也是每个人应尽的义务和职责。
我们应该大力发展绿化,增加森林面积,涵养水源。尽量使用节水器具,广泛宣传节约用水的好处,使人们自觉认识到水是珍贵的资源,摈弃“取之不尽,用之不竭”的陈腐观念。
为了创造美好的环境,我们要珍惜每一滴水。让我们从身边小事做起,从自己做起,保护有限的水资源,保护人们赖以生存的环境。我希望人们能拿出实际行动来弥补过去的过失。
Mother put a bamboo flowers at home, every weekend I go to water it This weekend, and the usual water, idle, I squatted down to observe carefully up the bamboo flowers
Bamboo flower stems like bamboo section by section, leaves like a long oval, very dark color of purple, some feel as if is black, and the leaves are alternate leaves (that is, side a, side a, asymmetric )
Black flowers grow very fast Remember three months ago, Zizhu spent just six point three cm, I in order to measure the altitude of the bamboo, I have to put on a bamboo stem, and draw scale Today, I looked carefully " ah, bamboo flowers grow quickly! " I was too shocked to call In three months time, Zizhu spent nearly twice as long, now quickly catch up with me in the bamboo stem high
Purple flowers are beautiful, but, open time is very short, only two days Flowers are deep pink, flower is yellow, and a little green, pink, yellow, very beautiful Its petals very small, but many, and several layers!
Bamboo blossom, better and better Mom said a bamboo flowers hope my study can be the same as the bamboo flowers on the rise
妈妈在家里插了一株紫竹花,我每个周末都去给它浇水。这个周末,和往常一样去浇水,闲着没事,我就蹲下身来仔细观察起紫竹花来了。
紫竹花的茎像竹子一样一截一截的,叶子像有点长的椭圆形,颜色非常深的那种紫,些得让人感觉仿佛是黑色的,且叶子是互生叶(就是这边一片,那边一片,不对称)。
紫竹花长得还很快。记得三个月前,紫竹花刚六点三厘米,我为了测量紫竹花的高度,我特意插上一根竹干,并在上划上刻度。今天,我仔细一看“啊,紫竹花长得好快呀!”我吃惊得叫到。三个月的时间,紫竹花几乎长了一倍,现在都快跟上我插的竹干高了。
紫竹花很漂亮,但是,开得时间很短,只有两天。花是深粉色的,花芯儿是的,还有一点绿,红里透黄,美丽极了。它的花瓣很小,但是很多,里外好几层呢!
竹子开花,节节高。妈妈说种上紫竹花希望我的学习也能像这紫竹花一样节节升高。
My bedroom
My bedroom is very clean and beautiful
Bedroom placed a big bookcase and a high chest, and a piece of bed I have read the book neatly on the shelf, I put the toys in the toy box, my best picture is me against the wall You look neat and tidy!
My bedroom is blue, with blue curtains, blue bed, blue table and a drawer, the most interesting is the blue wardrobe door on soldiers, horses, Shi and other chess pieces
我的卧室
我的卧室很整洁漂亮。
卧室里摆放着一个大大的书柜和一个个高高的衣柜,还有一张床。我把看过的书整齐地放在书架上,我把玩具整理在玩具箱内,我最好的画被我贴在墙上。你看多整洁呀!
我的卧室主要是蓝色的,有蓝色的窗帘,蓝色的床,蓝色的桌子和抽屉,最有趣的是蓝色衣柜门上还贴着兵、马、仕等象棋子。
My low carbon life
I am a economical, save a lot of examples One time, I saw my mother finished washing things the tap is not closed, I hurried past, turn the tap off, because I save water Another time, after I get up I saw the night open air conditioning, cost a lot of electricity, I quickly took the air conditioner remote controller for air conditioning, immediately shut down because I want to save electricity The day before yesterday, my father and I went to dinner, see all disposable chopsticks, when having a meal, I do not use disposable chopsticks, spoon, also advised father to also use the spoon, for protecting the environment of the earth to begin from me You asked me why I save, I just tell you that this is my low carbon life
我的低碳生活
我是一个节约的人,节约有很多事例。有一次,我看见妈妈洗完东西水龙头没关紧,我急忙跑过去,把水龙头给关了,因为我要节约用水。还有一次,我起床后看见空调连夜开着,耗费了很多电,我赶紧把空调遥控器拿来,立马关闭了空调因为我要节约电。前天,我和爸爸去吃饭,瞧见全部都是一次性筷子,吃饭时,我不用一次性筷子,用瓢羹,还劝爸爸也用瓢羹,因为保护地球环境要从我做起。你问我为什么要节约,我只告诉你这是我的低碳生活。
I love my mother My mother this year 38 years old, in CP Hua Secondary School teaching My mother long with a tall, round face always with a smile
Mother takes good care of me in life Get up at six thirty every morning, it woke me up, my mother gave me cooking, cooking, and hurried to school She always left me delicious, she often gives me a fish to eat, always give me delicious food, regardless of their own
Mother not only care about in life I, still learning patiently taught me, every night mother together with me to learn, I do my homework, mother preparing lessons, sometimes we both endorsed, story
I must study hard and make progress every day, grow up will repay mother
我最喜欢的人是我妈妈。我妈妈今年38岁,在茂华中学教书。我妈妈长着高高的个子,圆圆的脸蛋儿上总是带着微笑。
妈妈在生活上细心地照料我。每天早上六点半起床,起来了把我叫醒,我起来了妈妈给我做饭,做完饭,就急急忙忙的跑到学校。她总是把好吃的留给我,她经常给我做鱼吃,每次都给我夹好吃的,不顾自己。
妈妈不但在生活中关心我,还在学习上耐心地辅导我,每天晚上妈妈都陪我一起学习,我做作业,妈妈备课,有时候我们俩一起背书,讲故事。
妈妈我一定会好好学习天天向上,长大以后一定会报答妈妈的。
Learning English takes time so if we want to learn it well, we should spend much time practicing using English every day Besides, we should listen to the teacher and take notes carefully in class Go over what is learnt regularly and finish doing our homework carefully If we have time,we'd better isten to English radio programmes, read English stories and newspapers, watch English films and TV programmes ,keep a diary in English If we make mistakes, correct them at once and try not to make the same mistakes What's more, we should look up new words in the dictionary before class and prepare each lesson carefully before class I believe if we work hard and have good ways of learning English, we will learn English well
学英语是要花费时间的,因此我们要花大量的时间每天练习使用英语。而且我们应该上课认真听讲、记笔记、经常复习我们所学的、认真完成作业。如果我们有时间,最好听英语广播、读英语故事和报纸、看英语和电视节目、用英语记日记。如果我们出错了,就要马上更改并尽力别再犯同样的错误。并且我们在课前要查字典认真预习。我相信如果我们努力学习并且有好的学英语方法,我们一定会学好英语的。
I had a happy summer holiday because I did many interesting things
I went to the beach and I swam in the seaI called my friend and played with themI visited my grandparents and ate much delicious food
Then I went to the zoo whit my sisterI was very happyBecause I saw a lot of animalsThey were very lovelySo I took photos of all animalsI liked the butterflies bestThey were colourful and beautifulMy sister liked it too
I had a wonderful summer holiday this year
我有一个快乐的暑假,因为我做了许多有趣的东西。
我去了海边,我在大海里游泳。我打电话给朋友和他们玩了。我去拜访我的祖父母和吃了许多美味的食物。
然后我与我的妹妹去了动物园。我非常高兴。因为我看见很多动物。他们很可爱。所以我拍照,所有的动物。还有我最喜欢的喜欢蝴蝶。他们是五彩缤纷的和美丽的。我的妹妹非常喜欢它们。
今年我有一个很棒的暑假。
Today, I did a science experiment, observed the ants love what
I put an apple core, sugar, salt, dead insects, white rice in a field of central, then squatted looked on 15 minutes later, after half an hour, an hour later, " bait ", still do not have a " scout" In my anger, after the nearby ants were caught on the " bait ", but they flew away I'm really disappointed!
My static under heart to carefully analyze the reasons: " bait " is " no one shows any interest in ", is most likely to be too far away from the colony Then, I put the " bait " moved to the colony near Before long, they came in flocks to climb over They are busy carrying food, but the ant colony and egoistic They do not move the food, only know to eat, but hard to eat, one can die! To tell the truth, I don't know what it is to die, just guess
However, sugar were moved, finished, other " bait " still " no one shows any interest in " Therefore, the ants love sugar
今天,我做了一个科学小实验,观察蚂蚁最爱吃啥?
我把苹果核、白糖、食盐、死了的昆虫、白米饭放在一块空地的中央,然后蹲在旁边观看。15分钟过去了,半个小时过去了,一个小时过去了,“诱饵”上仍然没有一个“侦察兵”过来。我一气之下,把经过附近的蚂蚁都抓来放在“诱饵”上,可是它们却飞也似地逃走了。真失望!
我静下心来仔细分析原因:“诱饵”之所以“无人问津”,极有可能是离蚁窝太远。于是,我把“诱饵”移到蚁窝附近。没过多久,蚂蚁就成群结对地爬过来了。它们忙着搬食物,但蚁群里也有自私自利的家伙。它们不搬食物,只知道吃东西,而且玩命地吃着,有一只还撑死了呢!说实话,我也不知道它是怎么死的,只是猜测啦。
然而,白糖全部被搬完、吃完了,其他“诱饵”仍然“无人问津”。所以,蚂蚁最爱吃糖。
This morning, sister by car come, also brought a child car We asked the mother 's consent To eighty meters avenue to play We took turns riding The younger sister to ride, she rode fast, like a hunted by the tiger, also like a fly fast the little bird Suddenly, I said: " sister, be careful, there is a built well, " sister startled, quickly stopped I run and run, and finally went to sister, breathlessly to say: " we put the cover on the right, " she laughed Agreed with me We tried hard to move up, and fell down, almost did not put down on us, but we do not lose heart, but moved up, before long, we will cover the well We got home, mum said angrily: "you how how do the clothes are all wet " we make it through one one tell mom, mom says we can not only serve for people, do the good thing, hearing these words, our sister Z 's heart seemed to eat honey, sweet, on the face also covered with happy smile
今天上午, 妹妹乘着汽车来了, 还带来了一辆小童车 我们征求了妈妈的同意到八十米大街去玩 我们轮流骑着车 该妹妹骑了, 她骑的飞快, 活象一个被追杀的老虎, 还象一只飞的飞快的小鸟 突然, 我说: “妹妹,小心, 那有个没盖的井,” 妹妹吓了一跳, 连忙停了下来 我跑啊跑, 终于跑到了妹妹跟前, 上气不接下气地说: “咱们把井盖盖上吧,” 她笑着同意了我的意见我们使尽全身力气搬了起来, 又掉了下去, 差点没把我们砸住, 可我们不泄气,又搬了起来, 没过多久, 我们就将井盖盖上了我们回到家里, 妈妈生气地说:“你们怎么搞的怎么衣服都湿了” 我们把事情的经过一一告诉了妈妈, 妈妈说我们不仅能为人民服务,还做了件好事呢, 听了这些话, 我们姐妹z的心里都好象吃了蜜一样, 甜滋滋的, 脸上也挂满了快乐的笑容
在日常生活或是工作学习中,大家都尝试过写作文吧,通过作文可以把我们那些零零散散的思想,聚集在一块。写起作文来就毫无头绪?以下是我为大家整理的初二英语作文10篇,希望对大家有所帮助。
初二英语作文 篇1Last Sunday, I went to see a robot show with my good friend There were many kinds of robots They could cook They could walk and dance And they could talk with people Some different robots could play some instruments
Some of them had huge heads And they looked like humans I thought they were so cool and I loved them very much I will be a scientist in the future Because I can make lots of robots They can help people do many things They can do the most unpleasant jobs
初二英语作文 篇2Nowadays, most people have their idols Their idols may be singers, actors, athletes and so on People can get or learn something from their idols I like swimming very much and my idols is a swimmer He is Sun Yang, the most famous and popular swimmer in our country He is the gold medalist of London Olympic Games He became the national icon last year, because he won the gold medal in Shanghai World Swimming Championships and broke the world record Expect for his excellent performance, Sun Yang is an attractive young man He has warm smile and many lovely expressions Besides, he sings well and he performs in many shows People speak highly of his performance
现在,大部分的人都有自己的偶像。他们的偶像可能是歌手,演员以及运动员等等。人们可以从他们的偶像那里得到或学到一些东西。我很喜欢游泳,而且我的偶像是一名游泳运动员。他就是孙杨,我国最出名和受欢迎的游泳运动员。他是伦敦奥运会的金牌得主。去年,他成为了国家的标志,因为他获得了上海世界游泳锦标赛的金牌并打破了世界纪录。除了他优秀的表现外,孙杨还是一位年轻有魅力的男人。他的笑容温暖,表情也很可爱。除此之外,他在很多节目唱歌表演。人们高度赞扬他的表现。
初二英语作文 篇3The old year passed, and the new year began Today is January 1st, it is a new year and a new day In the evening, I saw many places to set up fireworks and make the whole sky dress up Colorful, piece by piece, like the ocean of flowers, is far away from my 25 floor, especially beautiful To the streets of Yingze, on the car like a long line, a row of moving forward, the beautiful light of the entire earth bright
The beginning of the new year, I have many plans, I want to Study hard, every day, every day, Study hard, ability to learn, to win glory for the country
初二英语作文 篇4A Diary
September 20th
Friday Rainy
At the beginning of the term, I made up my mind to learn English well in my plan Then I carried out the plan full of determination I stu-died as hard as I could,and so the days and weeks went by Soon the time for the mid-term exam came I went over my lessons in earnest,because Iexpected to get good marks But to my surprise, I got a low mark of 73I made quite a few grammar mistakes Besides, I was unable to use some words or idioms correctly I felt very sorry about it I sat in the classroom in low spirits
Knowing about it,my English teacher asked me to go to his of-fice and told me not to be so disappointed He also told me to listen to the teacher attentively in class,to make notes while listening and to do more reading and writing,and that only in this way could I learn Eng-lish well
What he said struck me greatly I am determined to do as my tea-cher told me to I'm sure I'll make some progress next time
参考译文:
日记:如何学好英语
9月20日 星期五 雨这个学期刚开始,我就在计划中下定决心学好英语。然后我充满信心地执行计划。我尽可能的努力学习,日子就这样一天天过去了,马上期中考试就到了。我认真地复习了这门课,因为我期望取得好成绩,但令我吃惊的是,我只得了73分,我犯了很多语法错误。而且,我不能正确的使用一些词和词组,我感到很难过,我坐在教室里,情绪很低。
知道这件事后,我的英语老师把我叫到他的办公室,叫我不要太失望了,他还告诉我上课要仔细听讲,边听边做笔记,多阅读,多写作,这样才能学好英语。
他说的话令我很感动,我决心按老师教的去做,我相信下次我将会有一些进步。
初二英语作文 篇5My father has small eyes,Wear a pair of glasses,looking more gently,the daddy's hair are not many, he said "the intelligent head does not grow hair",daddy does the management, but daddy usually ever not talk bureaucratically to others, therefore his personal connection is specially good,is the same as medaddy speaks always extremely temperately,never say hit the person,curse at people are also few,calculated scolded people not to be big, although sometimes also can be angry for a longtime,but just only about one day,could no surpass in one day-long
He usually educate me that the person must to be honestly,friendly to other,and working diligentlyThis is my father, I love him forever
我的父亲有一双小小的眼睛,戴着一副眼镜,看上去很温和,爸爸的头发并不多,他说“聪明的脑袋不长头发”,父亲是做管理工作的,但爸爸通常不曾专横地跟他人说话,因此,他的人际关系特别好,和我一样。爸爸说话总是十分温和地说话,从不说打击任何人,也很少咒骂别人,虽然有时也可以为其生气一段时间,但是仅仅只有一天,绝为会超过一天。
他通常教育我,人必须诚实,友好,工作努力。这是我的父亲,我永远爱他。
初二英语作文 篇6In 10 years, I think I will be a reporter I will live in Hong Kong,because I went to Hong Kong last week and fell in love with itI thinkHong Kong is a very beautiful city As a reporter, I willmeet lots ofinteresting people I think I’ll live in an apartment with my best friends,because I don’t like living alone During the week, I’ll look smart
初二英语作文 篇7My father likes fishing He always goes fishing with his friends in Autumn
One day, he took me to fish, too In autumn, fish have grown up It is the best season to fish First, we chose a good place Then my father helped me put an earthworm onto the fishhook Afterwards, I threw the fishhook into the river I waited for a long time Suddenly, I saw the float sink I was very happy I pulled it out of the water but I only saw half of the earthworm The fish played a trick on me I was very angry But my father said to me, “it takes time You need to be more patient” I tried again A few minutes later, I caught a big fish and I showed it to my father My father praised me, “what a good angler you are!”
我父亲喜欢钓鱼。秋天他总是和他的朋友去钓鱼。
有一天,他带我去钓鱼。秋天,鱼已经长大了。秋天是钓鱼最好的季节。首先,我们选择了一个好地方。然后我的父亲帮我把一个蚯蚓在鱼钩上。后来,我把鱼钩扔进河里。我等了很长时间。突然,我看到了浮子下沉。我非常高兴。我把它从水里,但我只看到一半的蚯蚓。鱼给我开了个玩笑。我很生气。但是我的父亲对我说,“这需要时间。你需要更多的耐心。“我再次尝试。几分钟后,我钓到了一条大鱼,我拿给我的父亲。爸爸表扬了我,“你有很好的耐心!”
初二英语作文 篇8I have many hobbies, such as playing computer games, reading comics, going shopping I also like playing pingpong very much I have a friend His name is Nick He is crazy about playing pingpong, too If we have free time, we often play pingpong together One summer holiday, he took me to a sports centre Then we played pingpong together, but he always lost He was very angry He said to me, "I will beat you in a month""OK We will have a match at that time"After a month, when I talked to him about it, he answered,"I forgot the thing! I don't want to have a match with you" I was very surprised and angry and said,"Oh, you're a forgetful boy!”
我有很多爱好,比如玩电脑游戏,看漫画,去购物。我也非常喜欢打乒乓球。我有一个朋友。他的名字叫尼克。他也喜欢玩乒乓球。如果我们有空闲时间,我们经常在一起打乒乓球。一个暑假,他带我去体育中心。然后我们一起玩乒乓球,但他总是输了。他很生气。他对我说,“我将在一个月内击败你。”“好吧。我们将进行一场比赛。“一个月后,当我和他说过话,他回答,“我忘了的东西!我不想和你进行一场比赛。“我非常惊讶和愤怒,说:“哦,你是一个健忘的男孩!”
初二英语作文 篇9Hello! My name is Allison Chen I born in China and I’m 13 years old I have long and dark hair, black and gorgeous eyes, small pinky lips and one dimple on each sides of my cheek My favourite colors are green and silver Sushi and Pizza are my favourite food I did well in school and I like almost every subjects
I’m a girly girl who loves shopping I like to dress up myself and make me look like a princess I have a huge closet that is full of clothes The reason I have so many clothes is that I couldn’t stop buying them once I stepped inside the mall Because of that, I drive my parents crazy, but they couldn’t help it Sad!
I will transform into a tomboy when I play sports One of my favourite sports is volleyball I love it! I always start playing volleyball with zeal, and end up with sweats It’s fun to play volleyball with other people小学作文 , and you shouldn’t be afraid of the ball because you think you might get hurt Don’t worry! You’ll be fine is you practice everyday
This is me-Allison Chen! And I like being myself If you like me, then please be my friend
初二英语作文 篇10One day in the summer vacation, I and dad after breakfast, came to the gan and fishing
I take out in advance to buy a good bait, wear on the hook, and threw the hook into the water Just for a minute or two, and I saw buoy sinking, there must be a fish took I hurriedly filed a fishing rod, saw a fish hook and sure enough, I hurried to catch the line, the hook from the fish's mouth out
Still entered into with water in a bucket, and then wear good bait on the hook, threw it into the water, such as fish bait quietly
But, after a few minutes, there is still no fish bait, I have to sit still, want to lift hook, it is, dad talked: "fishing to be patient, it is can't catch fish" I hear the word of the father, continue to wait But more than ten minutes passed, and still no fish bait So dad told me to change a place to catch, but still no fish bait, how to return a responsibility I mentioned a look, originally the bait has been eaten by the slippery fish, I have to replace bait, in a short time, a fish bait I asked my father: "why just down the earthworm will cause the fish" Dad replied, "just down the earthworms have a kind of smell, fish smelled the odor will know that there are food, can swim by"
可参考:诱饵基因转化筛库宿主菌Y1901) 3 个1mm 克隆接种于3mlYPD 液体培养基30℃振荡培养20 小时(过夜),
OD600 达到12 左右。
2) 将此3ml 菌液接入大体积(体积视转化个数定)YPD 中培养4 小时左右,使
培养液OD600 达到06。
3) 50ml 离心管收集菌液(Falcon, BD),Eppendorf Centrifuge 5810R, 700g (1,
865rpm),5 分钟离心收集菌体,弃上清。
4) 20ml ddH2O 重悬菌体,Eppendorf Centrifuge 5810R,700g,5 分钟离心收
集菌体,弃上清。
5) 20ml 1XTE/LiAc重悬菌体,Eppendorf Centrifuge 5810R ,700g,5 分钟离
心收集菌体,弃上清。
6) 根据每个转化需要50μl 菌液的量加入1XTE/LiAc 重悬菌体,分装到
Eppendorf 管中,每管50μl 菌液。
7) 每管加约100ng Bait 基因质粒,5μl 预变性的Carrier DNA,500μl 1X
TE/LiAc/PEG。
8) 放置于30℃培养箱或者水浴中孵育30 分钟,15 分钟时混匀一次。
9) 30 分钟后,每管加入15μl DMSO(DIMETHYL SULFOXIDE),轻轻上下颠
Bait 基因的自激活检测
一般采用膜检的方法,检测LacZ 基因的表达情况。膜检方法如下:
1) 将阳性克隆菌落影印于滤纸上。
2) 将滤纸完全浸于液氮中,时间长于30 秒,取出后晾干。
3) 培养皿加入适量显色液(新鲜配制),垫一层滤纸,让其完全湿润,将冻溶
后的滤纸铺在上面,使显色液渗透到表层。9cm 培养皿加2ml 显色液,15cm
培养皿加5ml 显色液。
4) 盖上皿盖,放置在37℃培养箱中,避光放置。
5) 20 分钟后开始观察有无变蓝的阳性结果并记录,一般以3 小时为准,特殊情
况下可以看过夜是否变蓝。
6)反应过后打开皿盖,将滤纸烘干,保存并记录。
35cycles
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